منابع مشابه
Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay.
BACKGROUND Mast cells are primary mediators of allergic inflammation. Antigen-mediated crosslinking of their cell surface immunoglobulin E (IgE) receptors results in degranulation and the release of proinflammatory mediators including histamine, tumor necrosis factor-alpha, and leukotrienes. METHODS Mast cells were stimulated to degranulate by using either IgE crosslinking or ionophore treatm...
متن کاملA novel flow-cytometric assay to assess neutrophil adhesion in whole blood.
740 the known susceptibility of HIBECs to lymphocyte-mediated damage. This report describes the development and application of a flow-cytometric assay for examination of the effects of cytokine stimulation on lymphocyte adhesion to monolayer cultured endothelial and epithelial cells. This assay is potentially superior to conventional isotopic features as it is less dependent on the structure of...
متن کاملFlow Cytometric Determination of Neutrophil CD64 (nCD64) in Children with Community Acquired Pneumonia
Background The expression of CD64 in neutrophils (nCD64) has shown utility in the diagnosis of sepsis. The aim of this study was to evaluate the diagnostic value of neutrophil CD64 in pneumonia as an early marker for infection and correlate its level with the outcome of...
متن کاملFlow cytometric kinetic measurements of neutrophil phospholipase A activation.
The interrelationships between activation of phospholipases and neutrophil stimulus-induced Ca2+ responses remain unclear. We report here that immune complexes activate a phosphatidylcholine-specific phospholipase A in a neutrophil only after the cytoplasmic Ca2+ transient has been initiated in the same cell, while chemotactic peptide activation does not proceed via such a phospholipase A-media...
متن کاملFlow cytometric assay for direct quantification of neutrophil extracellular traps in blood samples.
Neutrophil extracellular traps (NETs) contribute to innate immunity as well as numerous diseases processes such as deep vein thrombosis, myocardial ischemia, and autoimmune disease. To date, most knowledge on NETs formation has been gathered via the qualitative microscopic examination of individual neutrophils in vitro, or aggregate structures in vivo. Here we describe a novel flow cytometry (F...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1983
ISSN: 0022-1554,1551-5044
DOI: 10.1177/31.6.6404983